Microscopy- Preparation of wet mounts :: Curriculum

Biotechnology Research and Development Model

Units:

Microscopy- Preparation of wet mounts

Part of Unit: Laboratory Techniques and Safety

Lesson Plan Overview / Details

Students will prepare wet mounts of cheek cells. At the end of the lesson, students will learn to prepare specimens, stain them and observe them at different magnifications

Microscopy- Preparation of wet mounts

Cheek cells: 50 Minutes

Standards

California Career and Technical Education Standards

HSMT.FS.11.0 Demonstration and Application

California Academic Content Standards (Reinforced)

ELA.8.R.CAGT.2.5 Understand and explain the use of a complex mechanical device by following techn...3
S.7.IE.7.a Select and use appropriate tools and technology (including calculators, computer...

Objectives and Goals

Students will prepare and stain cheek cells to observe under compound microscope

Activities in this Lesson

Introduction to Microbiology - Lecture
- Microbes [ Download ]
- Module5 Folder.zip [ Download ] Cleaning Microscope slides and Preparing a Wet Mount
Preparation of bacterial smear - Demo / Modeling
Show the students how to do a bacterial smear, and observe under a microscope. Students will try it a few times. This will be a pre lab for gram staining.
Wrap-up - Closure
Follow safety procedures to discard microscope slides and cover slips into broken glass containers

Tooth picks used for smearing bacterial should be discarded in biohazard waste

Security officers will check the lab stations for any violations
- Violations Entry.pdf [ Download ] Security Officer Form