Part of Lesson Plan: Biofuel Enzyme Kit

Activity Overview / Details

Students should have already done a pre-lab or prepared for the lab prior to the start.  Have students flow chart the steps or write out the procedure for homework or during class the day before.  Students may also complete pre-lab questions directly from the Biorad Biofuels kit (pages 40-41 of the document below).

Students should be working in lab groups of 2-3 per group.

Briefly model the procedures for Activity 1: Determine the Reaction Rate in the Presence or Absence of an Enzyme (pages 42-45).  Teachers may build in stop points to ensure students are on similar steps.  To do this, model steps #1-7 and let students go.  Once the class has completed those steps, model steps #8-12 and have the class perform them.

Depending on class time, have students either clean up the lab or complete the qualitative and quantitative determination of product formed (pages 45-48).  I highly suggest performing hte first graph (page 47) together as a class and having students perform the second graph (page 48) in their lab pairs.  The calculation and/or graphs may be done at the start of class the following day.