Biotechnology Research and Development Model
Units:
- The Role of Biotechnology
- Making Cheese
- Careers in Genetic Technology
- DNA Origami
- Lights, Camera, Action! The Biotech Experience: Introduction
- Making Apple Juice
- Lights, Camera, Action! The Biotech Experience: Storyboarding
- Lights, Camera, Action! The Biotech Experience: Filming
- Lights, Camera, Action! The Biotech Experience: Post-Production
- Web Page Design in Biotechnology
- Researching Biotechnology Companies for the Purpose of Investment
- Investing in Biotechnology Companies
- Root Beer Making
- How to Set Up a Legal Scientific Notebook
- Current Events in Biotechnology
- Mathematical and Scientific Concepts
- Getting to know each other! Our phenotypes and genotypes
- Metric Measurements
- Using C1V1=C2V2
- DNA Ornament
- Solutions, Measurements and Data Analysis - Olson
- Chromosomes and the Human Genome - Olson
- Mapping Central Dogma
- Antibodies
- Ouchterlony Test: Antibody Simulation
- Understanding Relative Sizes in Worlds We Don't See
- Biofuel Enzyme Kit
- Plant Tissue Culture
- Why Clones?
- Preparation
- Explants
- Maintenance of Clones
- Biotechnology Components and Fields
- Genetic Engineering with Bio-Rad pGLO
- Recombinant DNA Paper Lab
- Bioprospecting and Herbicide Development
- Environmental Microbiology and development of antibiotics
- Biotechnology Career Research Project
- Understanding Sequencing
- Bioinformatics - Primer Design
- Spectrophotometric Analysis of Phytoplankton Growth
- Laboratory Techniques and Safety
- Biotechnology Lab Equipment
- Micropipette Use
- Loading a Gel
- DNA Extraction Lab
- Polymerase Chain Reaction - Pre Lab (Part 1)
- Polymerase Chain Reaction - Virtual Lab (Part 2)
- Polymerase Chain Reaction - Lab
- Epidemiology Lab - Viral Transmission
- Microscopy- Observing Prepared Slides
- Sterile Transfer Techniques/ Starting a Broth Culture
- Microscopy- Preparation of wet mounts
- Microscopy- Gram Staining Techniques
- Microscopy- Introduction
- Chromatography
- How to Make LB Agar
- Plant Extraction Lab
- Intro to Gel Electrophoresis Lab
- DNA Gel Electrophoresis Lab
- Liquid Media/ Bacteria Identification
- Why Aseptic Technique?
- Bacteria Isolation/ Streak Plates
- The Effect of Hormone Concentration on Plant Propagation
- Plant as a gift
- Effect of Hormone Concentration on Plant Propagation Lab
- Day 1 Filter Rootone
- Day 2 Lecture and Serial Dilution
- Day 3 Make Fuchsia holding containers
- Day 4 Make Data Tables
- Students observe or record data daily or weekly
- Analyze Graphs and Conclusion
- Students plant successful fuchsias and give out as gifts.
- Student Safety Video by Students
- Pipette Pointillism
- Product Design, Procedures, Licensure and Regulations
- Ethical, Moral, Legal, and Cultural Issues
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Activity Originally Created By:
Tina Doss
Maintenance of Clones
Part of Lesson Plan: Plant Tissue Culture
Activity Overview / Details
Part three is something that can be done as closure to the lab. Students can perform steps #15-21 (pages 204-205). One way to motivate students (if needed) is to tell them that they will have multiple african violets to give away as presents for the holiday season (I start the lab in September to make sure the lab is completed in time for the holidays). In this portion of the lab, students make more media and cut apart their explants into separate plants. This can only be done if there is individual plantlets (tiny roots and leafs) on the explants. They put the plantlets into the new medium, rich in nutrients for root and leaf growth. When the plantlets are large enough, students can plant into little styrofoam cups with soil.
Teacher note: I have students add a paragraph briefly explaining the science and procedures that it took to make the new african violet. They decorate the cups, attach the paragraph and hand the new plants out for the holidays to staff and family.




