How to Make LB Agar



LB (Luria-Bertani) Agar is an essential media in using for bacteria growth. This lesson has students solve the ratio equation for how much LB agar to measure out. Then students dissolve the agar using a hot plate and store it in a media bottle. Finally students place their media bottle in an autoclave to sterilize.

Lesson Time

Two Class Periods
50 Minutes

Industries / Subjects / Grades

Industries / Pathways
  • Health Science and Medical Technology

Standards and Objectives


California Academic Content Standards (4)
California's 2008 CTE Standards (3)

Related Instructional Objectives (SWBAT...)

  • Students will learn proper procedures in using lab equipment to obtain a sterile environment.
  • Students will figure out the amount of LB agar to measure out using using a ratio equation with the directions on the bottle.
  • Students will analyze the success of their sterile technique by looking for contamination.

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